Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025)

Name: Anti-Fibrinogen Alpha Chain Antibody [ARC2227]
Brand: Antibodies.com
SKU: A308025
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2227] antibody to Fibrinogen Alpha Chain for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Fibrinogen Alpha Chain Antibody [ARC2227]

Description

Rabbit monoclonal [ARC2227] antibody to Fibrinogen Alpha Chain.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human Fibrinogen alpha chain (FGA) (FGA) (P02671).

Sequence

NDEGEGEFWLGNDYLHLLTQRGSVLRVELEDWAGNEAYAEYHFRVGSEAEGYALQVSSYEGTAGDALIEGSVEEGAEYTSHNNMQFSTFDRDADQWEENCA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2227

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

95 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ac1873, Fba5e, FGA, Fib, Fib2, FIBA_HUMAN, Fibrinogen, A alpha polypeptide, Fibrinogen--alpha polypeptide chain, Fibrinopeptide A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025)

Western blot analysis of extracts of various cell lines, using Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Fibrinogen Alpha Chain Antibody [ARC2227] (A308025) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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