Anti-Fibrillarin Antibody [ARC0506] (A306505)

Name: Anti-Fibrillarin Antibody [ARC0506]
Brand: Antibodies.com
SKU: A306505
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0506] antibody to Fibrillarin for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Nucleolar Marker Ribosomal Marker

Specifications

Name

Anti-Fibrillarin Antibody [ARC0506]

Description

Rabbit monoclonal [ARC0506] antibody to Fibrillarin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 222-321 of human Fibrillarin/U3 RNP (P22087).

Sequence

KYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0506

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

34 kD nucleolar scleroderma antigen, 34 kDa nucleolar scleroderma antigen, Fbl, FBRL_HUMAN, FIB, FIB1, FLRN, Histone-glutamine methyltransferase, Nop1p, RNA U3 small nucleolar interacting protein 1, RNU3IP1, rRNA 2' O methyltransferase fibrillarin, rRNA 2'-O-methyltransferase fibrillarin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Fibrillarin Antibody [ARC0506] (A306505)

Western blot analysis of extracts of various cell lines, using Anti-Fibrillarin Antibody [ARC0506] (A306505) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Fibrillarin Antibody [ARC0506] (A306505)

Western blot analysis of extracts of various cell lines, using Anti-Fibrillarin Antibody [ARC0506] (A306505) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Immunofluorescence - Anti-Fibrillarin Antibody [ARC0506] (A306505)

Confocal imaging of U-2OS cells using Anti-Fibrillarin Antibody [ARC0506] (A306505), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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