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FGFR2 Cell Based ELISA Kit (A103092)

Western Blot - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.com Protocol Diagram - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.com Protocol Summary - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.com Western Blot - Control Antibody - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.com
$550
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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Email
orders@antibodies.com

Name
FGFR2 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This FGFR2 Cell Based ELISA Kit allows for the detection of FGFR2 and the effects that certain stimulation conditions have on FGFR2 expression in different cell lines. Qualitative determination of FGFR2 concentration is achieved by an indirect ELISA format. In essence, the FGFR2 is captured by Anti-FGFR2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this FGFR2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
bacteria-expressed kinase, BBDS, BEK, BEK fibroblast growth factor receptor, BFR1, CD332, CD332 antigen, CEK3, CFD1, Craniofacial dysostosis 1, ECT1, FGF receptor, FGFR 2, FGFR-2, FGFR2_HUMAN, Fibroblast growth factor receptor 2, Hydroxyaryl protein kinase, Jackson Weiss syndrome, JWS, K SAM, K-sam, Keratinocyte growth factor receptor, Keratinocyte growth factor receptor 2, KGFR, KSAM, protein tyrosine kinase, receptor like 14, soluble FGFR4 variant 4, TK14, TK25
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.comEnlarge Image
Figure 1: Western Blot - FGFR2 Cell Based ELISA Kit (A103092)
Western blot analysis of extracts from A549 cells, using Anti-FGFR2 Antibody.
Protocol Diagram - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.comEnlarge Image
Figure 2: FGFR2 Cell Based ELISA Kit (A103092)
A diagram showing the protocol for FGFR2 Cell Based ELISA Kit.
Protocol Summary - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.comEnlarge Image
Figure 3: FGFR2 Cell Based ELISA Kit (A103092)
A summary of the protocol for FGFR2 Cell Based ELISA Kit.
Western Blot - Control Antibody - FGFR2 Cell Based ELISA Kit (CB5264) - Antibodies.comEnlarge Image
Figure 4: Western Blot - FGFR2 Cell Based ELISA Kit (A103092)
The mouse monoclonal antibody to GAPDH is used as a positive control inFGFR2 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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