Anti-FGF21 Antibody [ARC53983] (A306217)

Name: Anti-FGF21 Antibody [ARC53983]
Brand: Antibodies.com
SKU: A306217
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53983] antibody to FGF21 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-FGF21 Antibody [ARC53983]

Description

Rabbit monoclonal [ARC53983] antibody to FGF21.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-209 of human FGF21 (NP_061986.1).

Sequence

HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53983

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FGF 21, FGF-21, FGF21_HUMAN, FGFL, Fibroblast growth factor 21, PRO10196, UNQ3115

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-FGF21 Antibody [ARC53983] (A306217)

Western blot analysis of extracts of various cell lines, using Anti-FGF21 Antibody [ARC53983] (A306217) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-FGF21 Antibody [ARC53983] (A306217)

Western blot analysis of extracts of Mouse liver, using Anti-FGF21 Antibody [ARC53983] (A306217) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-FGF21 Antibody [ARC53983] (A306217)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-FGF21 Antibody [ARC53983] (A306217) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-FGF21 Antibody [ARC53983] (A306217)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-FGF21 Antibody [ARC53983] (A306217) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.