Anti-FGF2 Antibody [ARC0618] (A308407)

Name: Anti-FGF2 Antibody [ARC0618]
Brand: Antibodies.com
SKU: A308407
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0618] antibody to FGF2 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-FGF2 Antibody [ARC0618]

Description

Rabbit monoclonal [ARC0618] antibody to FGF2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FGF2 (NP_001997.5).

Sequence

RAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0618

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa / 22 kDa / 31 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Basic fibroblast growth factor, Basic fibroblast growth factor bFGF, BFGF, FGF 2, FGF B, FGF-2, FGF2 basic, FGF2_HUMAN, FGFB, Fibroblast growth factor 2, Fibroblast growth factor 2 (basic), Fibroblast growth factor, basic, HBGF 2, HBGF-2, HBGF2, HBGH 2, HBGH2, Heparin binding growth factor 2 precursor, Heparin-binding growth factor 2, Prostatropin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-FGF2 Antibody [ARC0618] (A308407)

Western blot analysis of extracts from wild type (WT) and FGF2 knockout (KO) HeLa cells, using Anti-FGF2 Antibody [ARC0618] (A308407) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-FGF2 Antibody [ARC0618] (A308407)

Western blot analysis of extracts of SKOV3 cells, using Anti-FGF2 Antibody [ARC0618] (A308407) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-FGF2 Antibody [ARC0618] (A308407)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-FGF2 Antibody [ARC0618] (A308407) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-FGF2 Antibody [ARC0618] (A308407)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-FGF2 Antibody [ARC0618] (A308407) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.