Anti-FGF1 Antibody (A12678)

Name: Anti-FGF1 Antibody
Brand: Antibodies.com
SKU: A12678
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to FGF1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-FGF1 Antibody

Description

Rabbit polyclonal antibody to FGF1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 16-155 of human FGF1 (NP_001138364.1).

Sequence

FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Acidic fibroblast growth factor, aFGF, Beta endothelial cell growth factor, Beta-endothelial cell growth factor, ECGF, ECGF beta, ECGF-beta, ECGFA, ECGFB, Endothelial Cell Growth Factor alpha, Endothelial Cell Growth Factor beta, FGF 1, FGF alpha, FGF1_HUMAN, FGFA, Fibroblast growth factor 1, Fibroblast Growth Factor 1 Acidic, GLIO703, HBGF 1, HBGF-1, HBGF1, Heparin binding growth factor 1, Heparin binding growth factor 1 precursor, Heparin-binding growth factor 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-FGF1 Antibody (A12678)

Western blot analysis of extracts of various cell lines, using Anti-FGF1 Antibody (A12678) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-FGF1 Antibody (A12678)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-FGF1 Antibody (A12678) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-FGF1 Antibody (A12678)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-FGF1 Antibody (A12678) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-FGF1 Antibody (A12678)

Immunofluorescence analysis of HepG2 cells using Anti-FGF1 Antibody (A12678) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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