Fez1 expression in Kelly cell lysate (A) + Peptide (B) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Fez1 Antibody (A82691) at 0.1µg/ml and detected by chemiluminescence.
Fez1 expression in Rat Brain lysate (A) + Peptide (B) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Fez1 Antibody (A82691) at 0.1µg/ml and detected by chemiluminescence.
Fez1 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Fez1 Antibody (A82691) at 5µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for Fez1 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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