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Anti-Fc epsilon RI/FCER1A Antibody (A85211)

Western Blot - Anti-Fc epsilon RI/FCER1A Antibody (A85211) - Antibodies.com Immunohistochemistry - Anti-Fc epsilon RI/FCER1A Antibody (A85211) - Antibodies.com Immunohistochemistry - Anti-Fc epsilon RI/FCER1A Antibody (A85211) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to Fc epsilon RI/FCER1A for ELISA, WB and IHC.

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$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-Fc epsilon RI/FCER1A Antibody
Description
Goat polyclonal antibody to Fc epsilon RI/FCER1A.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:128,000, WB: 0.3-1µg/ml, IHC: 6µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region, amino acids 177-189, of human Fc epsilon RI/FCER1A.
Sequence
C-TGKVWQLDYESEP
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
50 kDa
Predicted MW
29.6kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Fc epsilon RI/FCER1A Antibody (A85211) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Fc epsilon RI/FCER1A Antibody (A85211)
Fc epsilon RI/FCER1A expression in Human Peripheral Blood Lymphocyte lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Fc epsilon RI/FCER1A Antibody (A85211) at 0.3µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-Fc epsilon RI/FCER1A Antibody (A85211) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Fc epsilon RI/FCER1A Antibody (A85211)
Fc epsilon RI/FCER1A expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Fc epsilon RI/FCER1A Antibody (A85211) at 6µg/ml and revealed with horseradish peroxidase (HRP).
Immunohistochemistry - Anti-Fc epsilon RI/FCER1A Antibody (A85211) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Fc epsilon RI/FCER1A Antibody (A85211)
Negative control for Fc epsilon RI/FCER1A expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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