Anti-Fatty Acid Synthase Antibody (A12631)

Name: Anti-Fatty Acid Synthase Antibody
Brand: Antibodies.com
SKU: A12631

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This product is discontinued

Anti-Fatty Acid Synthase Antibody (A12631) has been discontinued and is no longer available.

View all Fatty Acid Synthase Antibodies.

Specifications

Name

Anti-Fatty Acid Synthase Antibody

Description

Rabbit polyclonal antibody to Fatty Acid Synthase.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2221-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).

Sequence

SLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

273 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FAS, FASN, Type I fatty acid synthase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Fatty Acid Synthase Antibody (A12631)

Western blot analysis of extracts of various cell lines, using Anti-Fatty Acid Synthase Antibody (A12631) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Fatty Acid Synthase Antibody (A12631)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Fatty Acid Synthase Antibody (A12631) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Fatty Acid Synthase Antibody (A12631)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Fatty Acid Synthase Antibody (A12631) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Fatty Acid Synthase Antibody (A12631)

Immunofluorescence analysis of U2OS cells using Anti-Fatty Acid Synthase Antibody (A12631) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).