Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer. Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.
Absorption Maxima
490 nm
Extinction Coefficient
80000 M-1cm-1
Emission Maxima
513 nm
Fluorescence Quantum Yield
0.93
CF260
0.20
CF280
0.17
Mass Spec M+ Shift after Conjugation
529.12
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C31H21N5O6
Molecular Weight
559.53 kDa
Product Form
Yellow solid.
Solubility
Good in DMF and DMSO.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
