Rabbit polyclonal antibody to FADD (Phospho-Ser190)
Specificity
The antibody detects endogenous levels of FADD only when phosphorylated at serine 190.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine190(N-R-S(p)-G-A) derived from Mouse FADD.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, MORT1
Western blot analysis of extracts from Jurkat cells treated with PMA using FADD (Phospho-Ser190) Antibody #11820.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser190) antibody #11820 (left)or the same antibody preincubated with blocking peptide (right).
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