Rabbit polyclonal antibody to Ezrin (Phospho-Tyr353)
Specificity
The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
81kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
CVIL, CVL, Cytovillin, Cytovillin 2, DKFZp762H157, Epididymis secretory protein Li 105, EZR, EZRI_HUMAN, FLJ26216, HEL S 105, MGC1584, p81, VIL 2, VIL2, Villin 2, Villin 2 (ezrin), Villin 2 ezrin, Villin-2, Villin2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353) Antibody #11063 (left) or the same antibody preincubated with blocking peptide (right).
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