Anti-Ext1 Antibody (A13822)

Name: Anti-Ext1 Antibody
Brand: Antibodies.com
SKU: A13822
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Ext1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Ext1 Antibody

Description

Rabbit polyclonal antibody to Ext1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human EXT1 (NP_000118.2).

Sequence

DNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4-alpha-N-acetylglucosaminyltransferase, exostoses (multiple) 1, Exostosin 1, Exostosin glycosyltransferase 1, Exostosin-1, EXT, EXT1_HUMAN, glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Langer-Giedion syndrome chromosome region, LGCR, LGS, Multiple exostoses protein 1, Multiple exostoses protein 1 homolog, N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, Putative tumor suppressor protein EXT1, TRPS2, TTV

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Ext1 Antibody (A13822)

Western blot analysis of extracts of various cell lines, using Anti-Ext1 Antibody (A13822) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Immunofluorescence - Anti-Ext1 Antibody (A13822)

Immunofluorescence analysis of HeLa cells using Anti-Ext1 Antibody (A13822) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Ext1 Antibody (A13822)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Ext1 Antibody (A13822) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Ext1 Antibody (A13822)

Immunofluorescence analysis of PC-12 cells using Anti-Ext1 Antibody (A13822) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).