Mouse monoclonal [CPTC-SULT1E1-1] antibody to Estrogen Sulfotransferase.
Specificity
The soluble sulfotransferases contribute to the elimination of xenobiotics, the activation of procarcinogens and the regulation of hormones by catalyzing the sulfate conjugation of these substances. SULT1E1, also known as STE, is a 294 amino acid member of the SULT1 family. Localized to the cytoplasm and expressed in intestine, liver and kidney, SULT1E1 exists as a homodimer that is thought to control estrogen receptor (ER) levels by sulfurylating free estradiol. Defects in the gene encoding SULT1E1 are associated with an increased risk for endometrial cancer, suggesting a role for SULT1E1 in tumorigenesis.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human SULT1E1 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
CPTC-SULT1E1-1
Isotype
IgG2b
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine using Anti-Estrogen Sulfotransferase Antibody [CPTC-SULT1E1-1].
SDS-PAGE analysis of Anti-Estrogen Sulfotransferase Antibody [CPTC-SULT1E1-1] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Figure 4: Western Blot - Anti-Estrogen Sulfotransferase Antibody [CPTC-SULT1E1-1] (A250068)
Western blot analysis of liver tissue lysate using Anti-Estrogen Sulfotransferase Antibody [CPTC-SULT1E1-1].
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