Anti-Ki67 Antibody [6G3] (A104345)

Immunofluorescent analysis of HeLa cells stained with Anti-Ki67 Antibody (1:2,000 | red). The blue is DAPI staining of nuclear DNA. The Anti-Ki67 Antibody stains Ki67 protein predominantly expressed in the nucleoli of rapidly dividing cells, while nearby quiescent cells express little or no Ki67. The Ki67 protein also coats the surface of condensed chromosomes as seen at the middle left of the image.

Western blot analysis of equal amounts of cell lysates using Anti-Ki67 Antibody (1:2,000 | green): [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of apparent molecular weight of 345 and 395kDa. Smaller proteolytic fragments of these isoforms are also invariably detected on the blot.