Anti-ERK5 Antibody [ARC0871] (A306729)

Name: Anti-ERK5 Antibody [ARC0871]
Brand: Antibodies.com
SKU: A306729
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0871] antibody to ERK5 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ERK5 Antibody [ARC0871]

Description

Rabbit monoclonal [ARC0871] antibody to ERK5.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 717-816 of human ERK5 (Q13164).

Sequence

LSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0871

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

115 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Big MAP kinase 1, BMK-1, BMK1, ERK-5, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, MAPK7, Mitogen-activated protein kinase 7, PRKM7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-ERK5 Antibody [ARC0871] (A306729)

Western blot analysis of extracts of various cell lines, using Anti-ERK5 Antibody [ARC0871] (A306729) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-ERK5 Antibody [ARC0871] (A306729)

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Immunohistochemistry - Anti-ERK5 Antibody [ARC0871] (A306729)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ERK5 Antibody [ARC0871] (A306729) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ERK5 Antibody [ARC0871] (A306729)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ERK5 Antibody [ARC0871] (A306729) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.