Anti-ERK1 + ERK2 Antibody (A92634)

Name: Anti-ERK1 + ERK2 Antibody
Brand: Antibodies.com
SKU: A92634
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ERK1 + ERK2 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ERK1 + ERK2 Antibody

Description

Rabbit polyclonal antibody to ERK1 + ERK2.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:2,000, ICC/IF: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAPK1/MAPK3 (NP_620407.1/NP_002737.2).

Sequence

LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 kDa / 43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK 1, ERK 2, ERK-2, ERK1, erk1/2, ERK2, ERT1, ERT2, Extracellular signal regulated kinase 1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Mapk1, MAPK2, MAPK3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01_HUMAN, p38, p40, p41, p42-MAPK, PRKM 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ERK1 + ERK2 Antibody (A92634)

Western blot analysis of extracts of various cell lines, using Anti-ERK1 + ERK2 Antibody (A92634) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-ERK1 + ERK2 Antibody (A92634)

Immunofluorescence analysis of NIH-3T3 cells using Anti-ERK1 + ERK2 Antibody (A92634) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-ERK1 + ERK2 Antibody (A92634)

Confocal immunofluorescence analysis of Hela cells using Anti-ERK1 + ERK2 Antibody (A92634) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).

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