Anti-ERK1 Antibody (A83709)

Name: Anti-ERK1 Antibody
Brand: Antibodies.com
SKU: A83709
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to ERK1 for ELISA, WB, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ERK1 Antibody

Description

Goat polyclonal antibody to ERK1.

Applications

ELISA, WB, IF, Flow Cytometry

Dilutions

ELISA: 1:4,000, WB: 0.1-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Cross Reactivity

This antibody is not expected to cross react with ERK2

Immunogen

Synthetic peptide corresponding to the N terminal region of human ERK1.

Sequence

GGEPRRTEGVGP-C

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

40 kDa

Predicted MW

40.1kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ERK, ERK 1, ERK-1, ERT 2, ERT2, Extracellular Signal Regulated Kinase 1, Extracellular signal related kinase, Extracellular signal related kinase 1, Extracellular signal-regulated kinase 1, HGNC6877, HS44KDAP, HUMKER1A, Insulin Stimulated MAP2 Kinase, Insulin-stimulated MAP2 kinase, MAP Kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK, MAPK 1, MAPK 3, MAPK1, Mapk3, MGC20180, Microtubule Associated Protein 2 Kinase, Microtubule-associated protein 2 kinase, Mitogen Activated Protein Kinase 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, MK03_HUMAN, OTTHUMP00000174538, OTTHUMP00000174541, p44 ERK1, p44 MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM 3, PRKM3, Protein Kinase Mitogen Activated 3

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ERK1 Antibody (A83709)

ERK1 expression in Human Cerebellum (A) and Frontal Cortex (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ERK1 Antibody (A83709) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-ERK1 Antibody (A83709)

ERK1 expression in A431 (A) and HepG2 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-ERK1 Antibody (A83709) at 1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-ERK1 Antibody (A83709)

ERK1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK1 Antibody (A83709) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-ERK1 Antibody (A83709)

ERK1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK1 Antibody (A83709) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.