Anti-Ephrin A1 Antibody [ARC1443] (A307223)

Name: Anti-Ephrin A1 Antibody [ARC1443]
Brand: Antibodies.com
SKU: A307223
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1443] antibody to Ephrin A1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Ephrin A1 Antibody [ARC1443]

Description

Rabbit monoclonal [ARC1443] antibody to Ephrin A1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ephrin A1 (P20827).

Sequence

MEFLWAPLLGLCCSLAAADRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1443

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B61, ECKLG, EFL 1, EFL1, EFNA 1, Efna1, EFNA1_HUMAN, EPH related receptor tyrosine kinase ligand 1, EPH-related receptor tyrosine kinase ligand 1, Ephrin-A1, Ephrin-A1, secreted form, EphrinA1, EPLG 1, EPLG1, Immediate early response protein B61, LERK 1, LERK-1, LERK1, Ligand of eph related kinase 1, OTTHUMP00000033242, OTTHUMP00000033271, secreted form, TNF alpha-induced protein 4, TNFAIP 4, TNFAIP4, Tumor necrosis factor alpha induced protein 4, Tumor necrosis factor alpha-induced protein 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Ephrin A1 Antibody [ARC1443] (A307223)

Western blot analysis of extracts of HepG2 cells, using Anti-Ephrin A1 Antibody [ARC1443] (A307223) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Ephrin A1 Antibody [ARC1443] (A307223)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Ephrin A1 Antibody [ARC1443] (A307223) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Ephrin A1 Antibody [ARC1443] (A307223)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Ephrin A1 Antibody [ARC1443] (A307223) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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