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EPHA1 Cell Based ELISA Kit (A103677)

Western Blot - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.com Protocol Diagram - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.com Protocol Summary - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.com Western Blot - Control Antibody - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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Name
EPHA1 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This EPHA1 Cell Based ELISA Kit allows for the detection of EPHA1 and the effects that certain stimulation conditions have on EPHA1 expression in different cell lines. Qualitative determination of EPHA1 concentration is achieved by an indirect ELISA format. In essence, the EPHA1 is captured by Anti-EPHA1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this EPHA1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
EPH, Eph receptor A1, EPH tyrosine kinase, EPH tyrosine kinase 1, EPHA1_HUMAN, Ephrin receptor Eph A1, Ephrin type A receptor 1, Ephrin type-A receptor 1, EPHT, EPHT 1, EPHT1, Erythropoietin producing hepatoma amplified sequence, Erythropoietin-producing hepatoma receptor, Esk, hEpha1, MGC163163, Oncogene EPH, Soluble EPHA1 variant 1, Soluble EPHA1 variant 2, Tyrosine protein kinase receptor EPH, Tyrosine-protein kinase receptor EPH
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.comEnlarge Image
Figure 1: Western Blot - EPHA1 Cell Based ELISA Kit (A103677)
Western blot analysis of extracts from COLO205 cells, using Anti-EPHA1 Antibody.
Protocol Diagram - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.comEnlarge Image
Figure 2: EPHA1 Cell Based ELISA Kit (A103677)
A diagram showing the protocol for EPHA1 Cell Based ELISA Kit.
Protocol Summary - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.comEnlarge Image
Figure 3: EPHA1 Cell Based ELISA Kit (A103677)
A summary of the protocol for EPHA1 Cell Based ELISA Kit.
Western Blot - Control Antibody - EPHA1 Cell Based ELISA Kit (CB5231) - Antibodies.comEnlarge Image
Figure 4: Western Blot - EPHA1 Cell Based ELISA Kit (A103677)
The mouse monoclonal antibody to GAPDH is used as a positive control inEPHA1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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