Anti-EpCAM Antibody [VU-1D9] (A281944)

Anti-EpCAM Antibody [VU-1D9]

Mouse monoclonal [VU-1D9] antibody to EpCAM.

This antibody recognises epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium

WB, IHC-P, IHC-Fr, Flow Cytometry, IP

Flow Cytometry: 1:50 - 1:100, IHC-P: 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human

HG9 cell line (small cell lung carcinoma).

Mouse

Monoclonal

VU-1D9

IgG1

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Approximately 38 kDa in HT-29 cell lysates

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD326 antibody, clone VU-1D9 recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium

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