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Anti-Eosinophil derived neurotoxin Antibody (A12426)

Western Blot - Anti-Eosinophil derived neurotoxin Antibody (A12426) - Antibodies.com Immunofluorescence - Anti-Eosinophil derived neurotoxin Antibody (A12426) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Eosinophil derived neurotoxin for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Eosinophil derived neurotoxin Antibody
Description
Rabbit polyclonal antibody to Eosinophil derived neurotoxin.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 28-161 of human RNASE2 (NP_002925.1).
Sequence
KPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTFLLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDNRDQRRDPPQYPVVPVHLDRII
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Ear4, EC=3.1.27.5, EDN, Eosinophil cationic type ribonuclease 4, MR 4, Non secretory ribonuclease, R14, R15, R5, Ribonuclease 2, Ribonuclease RNase A family 2 (liver eosinophil derived neurotoxin), Ribonuclease US, RNase 2, RNase UpI 2, RNASE2, RNS2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Eosinophil derived neurotoxin Antibody (A12426) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Eosinophil derived neurotoxin Antibody (A12426)
Western blot analysis of extracts of various cell lines, using Anti-Eosinophil derived neurotoxin Antibody (A12426) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Eosinophil derived neurotoxin Antibody (A12426) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Eosinophil derived neurotoxin Antibody (A12426)
Immunofluorescence analysis of mouse spleen using Anti-Eosinophil derived neurotoxin Antibody (A12426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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