Anti-eNOS (phospho Thr495) Antibody (A10996)

Name: Anti-eNOS (phospho Thr495) Antibody
Brand: Antibodies.com
SKU: A10996
Price: 450.0 USD
Availability: InStock

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$450

Rabbit polyclonal antibody to eNOS (phospho Thr495) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-eNOS (phospho Thr495) Antibody

Description

Rabbit polyclonal antibody to eNOS (phospho Thr495).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T495 of human eNOS (NP_000594.2).

Sequence

KKTFK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase, endothelial, NOS type III, NOS3, NOSIII

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-eNOS (phospho Thr495) Antibody (A10996)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-eNOS (phospho Thr495) Antibody (A10996) at 1:1,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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