Anti-eNOS (phospho Ser1177) Antibody (A16643)

Name: Anti-eNOS (phospho Ser1177) Antibody
Brand: Antibodies.com
SKU: A16643
Price: 450.0 USD
Availability: InStock

Enlarge Image

$450

Rabbit polyclonal antibody to eNOS (phospho Ser1177) for WB.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-eNOS (phospho Ser1177) Antibody

Description

Rabbit polyclonal antibody to eNOS (phospho Ser1177).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).

Sequence

TQSFS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

133 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase, endothelial, NOS type III, NOS3, NOSIII

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Enlarge Image

Western Blot - Anti-eNOS (phospho Ser1177) Antibody (A16643)

Western blot analysis of extracts of Jurkat cells, using Anti-eNOS (phospho Ser1177) Antibody (A16643). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

Enlarge Image

Western Blot - Anti-eNOS (phospho Ser1177) Antibody (A16643)

Western blot analysis of extracts of HeLa cells, using Anti-eNOS (phospho Ser1177) Antibody (A16643) at 1:1,000 dilution or eNOS antibody (A1548). HeLa cells were treated by IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

Publishing research using Anti-eNOS (phospho Ser1177) Antibody (A16643)? Please let us know so that we can list the citation on this page.