Anti-eNOS (phospho Ser1177) Antibody (A10995)

Name: Anti-eNOS (phospho Ser1177) Antibody
Brand: Antibodies.com
SKU: A10995
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to eNOS (phospho Ser1177) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-eNOS (phospho Ser1177) Antibody

Description

Rabbit polyclonal antibody to eNOS (phospho Ser1177).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).

Sequence

TQSFS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

133 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

cNOS, Constitutive NOS, EC NOS, EC-NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, Nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, NOS3, NOS3_HUMAN, NOSIII

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-eNOS (phospho Ser1177) Antibody (A10995)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-eNOS (phospho Ser1177) Antibody (A10995) at 1:1,000 dilution. NIH/3T3 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-eNOS (phospho Ser1177) Antibody (A10995)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-eNOS (phospho Ser1177) Antibody (A10995) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-eNOS (phospho Ser1177) Antibody (A10995)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-eNOS (phospho Ser1177) Antibody (A10995) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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