Anti-eNOS Antibody [ARC51618] (A306395)

Name: Anti-eNOS Antibody [ARC51618]
Brand: Antibodies.com
SKU: A306395
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC51618] antibody to eNOS for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-eNOS Antibody [ARC51618]

Description

Rabbit monoclonal [ARC51618] antibody to eNOS.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1110 of human eNOS (NP_000594.2).

Sequence

KGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51618

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

148 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase, endothelial, NOS type III, NOS3, NOSIII

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-eNOS Antibody [ARC51618] (A306395)

Western blot analysis of extracts of various cell lines, using Anti-eNOS Antibody [ARC51618] (A306395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-eNOS Antibody [ARC51618] (A306395)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-eNOS Antibody [ARC51618] (A306395) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-eNOS Antibody [ARC51618] (A306395)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-eNOS Antibody [ARC51618] (A306395) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-eNOS Antibody [ARC51618] (A306395)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-eNOS Antibody [ARC51618] (A306395) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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