Western blot analysis of extracts of 293T cells, using Anti-eNOS Antibody (A91724) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Western blot analysis of extracts of Mouse liver, using Anti-eNOS Antibody (A91724) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-eNOS Antibody (A91724) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HUVEC cells using Anti-eNOS Antibody (A91724) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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