Anti-ELMO2 Antibody (A83692)

Name: Anti-ELMO2 Antibody
Brand: Antibodies.com
SKU: A83692
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to ELMO2 for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ELMO2 Antibody

Description

Goat polyclonal antibody to ELMO2.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 0.3-1µg//ml, IHC: 3-6µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human ELMO2.

Sequence

C-PKEPSSYDFVYHYG

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

80 kDa

Predicted MW

82.6kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CED 12, Ced 12 homolog 2, CED 12 homolog A, CED 12A, CED-12, ced-12 homolog 2, CED12, CED12 homolog A, CED12, C. elegans, homolog of, 2, CED12A, ELMO 2, ELMO-2, ELMO2_HUMAN, Engulfment and cell motility 2, Engulfment and cell motility 2 (ced 12 homolog, C. elegans), Engulfment and cell motility gene 2, Engulfment and cell motility protein 2, FLJ11656, hCED 12A, hCED-12A, hCED12A, KIAA1834, OTTHUMP00000031762, PH domain protein CED12A, Protein ced 12 homolog A, Protein ced-12 homolog A

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-ELMO2 Antibody (A83692)

ELMO2 expression in Jurkat (A), HEK293 (B), RAW2647 (C), and KNRK (D) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ELMO2 Antibody (A83692) at 0.5µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-ELMO2 Antibody (A83692)

ELMO2 expression in Human Cerebellum (A) and Rat Brain (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ELMO2 Antibody (A83692) at 0.5µg/ml and detected by chemiluminescence.

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Figure 3: Immunohistochemistry - Anti-ELMO2 Antibody (A83692)

ELMO2 expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ELMO2 Antibody (A83692) at 3.8µg/ml and revealed with alkaline phosphatase (AP).

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Figure 4: Immunofluorescence - Anti-ELMO2 Antibody (A83692)

ELMO2 expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ELMO2 Antibody (A83692) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.