90 Minute ELISA Kits

By Christopher David PhD and Ryan Hamnett, PhD

Our 90 Minute ELISA Kits produce accurate, reproducible results in half the time of traditional ELISAs. They are simple to use, involving just one wash step, and require only a fraction of the sample volume demanded by conventional kits. With no compromise on specificity or sensitivity, and with over 4,000 possible tests to choose from, our 90 Minute ELISA Kits give you consistent results at an affordable price.

  • Complete assays in 90 minutes
  • Specific, sensitive, reproducible
  • Reduced reagent and instrument use
  • Easy to use
  • Wide selection of target antigens
  • Bulk purchase opportunities

Table of Contents

How the 90 Minute ELISA works

The enzyme-linked immunosorbent assay (ELISA) has become the standard method for in vitro quantification of target antigens in biological fluids, thanks to the high specificity of the antigen-antibody interaction. This is particularly relevant in sandwich ELISAs, in which matched pairs of antibodies bind the antigen at two distinct sites, increasing specificity.

Our 90 Minute ELISA Kits maintain this high level of precision while cutting down the number of steps, reducing the time spent washing, and using less of your precious sample.

Diagram of 90 minute ELISA workflow in 7 steps

Diagram created with BioRender.

  1. Users add standards and test samples to the wells of a 96-well microtiter plate, which is pre-coated with specific, validated antibodies.
  2. The antigen of interest present in each sample binds to the immobilized capture antibody.
  3. A biotinylated detection antibody that binds to the captured antigen is added, followed by streptavidin-HRP.
  4. During a 60-minute incubation, streptavidin-HRP binds to the biotinylated antibody, and the biotinylated antibody binds to the antigen being held by the immobilized antibody.
  5. After the incubation, unbound sample, unbound biotinylated antibody and unbound streptavidin-HRP are removed by 5 washes (also suitable for automated plate washing).
  6. A substrate solution is added to the wells, and colour develops in proportion to the amount of antigen captured in each well.
  7. The reaction is stopped by adding a stop solution, which changes the color of the solution from blue to yellow. Color intensity in each well is read using a plate reader.

Why choose the Antibodies.com 90 Minute ELISA Kit?

One of the primary concerns in any assay is sensitivity. Researchers often face a trade-off between achieving optimal sensitivity, the time required for the assay, and cost. Our 90-minute ELISA breaks this barrier, delivering optimal sensitivity at an affordable price without compromising on speed. This allows you to allocate more time to what truly matters – interpreting results and designing the next phase of your experiment. By reducing the assay time, we empower researchers to be more productive and focus on the creative aspects of their work.

90 Minute ELISA Kit Advantages What it means for you
High specificity, reproducibility and sensitivity High performance, every time
Time efficiency Complete high throughput assays in 90 minutes for research acceleration
Cost effective Cheaper than competitors without compromising on quality
Extensive catalogue Kits to measure 4,346 targets, over 3x more than any other supplier, across a wide range of research areas for both human and mouse samples
Resource optimization Less sample needed than conventional ELISAs and competitors’ kits
Flexible scheduling Plan experiments efficiently for tight schedules

90 Minute ELISA for drug discovery

The measurement of soluble factors is ubiquitous among the many stages of the drug discovery development process, and users spanning academia, biotech, pharma, and bio-pharma are positioned to benefit from the advantages that the 90 Minute ELISA offers over competing products.

Our extensive catalogue of over 4000 antigens means you can have consistent results across all of your experiments, whether you’re investigating a single target or an entire pathway.

Schematic of the drug discovery process, and how 90 minute ELISAs can contribute to each stage

Diagram created with BioRender.

Our assays undergo rigorous testing to ensure accuracy, reliability, and reproducibility. When you choose our technology, you're not just saving time – you're investing in results you can trust.

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