Anti-eIF5A Antibody (A13810)

Name: Anti-eIF5A Antibody
Brand: Antibodies.com
SKU: A13810
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to eIF5A for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-eIF5A Antibody

Description

Rabbit polyclonal antibody to eIF5A.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human eIF5A (NP_001137234.1).

Sequence

MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1, eIF4D, EIF5A1, eIF5AI, Eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A 1, Eukaryotic translation initiation factor 5A-1, IF5A1_HUMAN, MGC104255, MGC99547, Rev binding factor, Rev-binding factor, uORF, uORF A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-eIF5A Antibody (A13810)

Western blot analysis of extracts of various cell lines, using Anti-eIF5A Antibody (A13810) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Immunohistochemistry - Anti-eIF5A Antibody (A13810)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-eIF5A Antibody (A13810) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.