Anti-eIF4G1 Antibody (A10162)

Name: Anti-eIF4G1 Antibody
Brand: Antibodies.com
SKU: A10162
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to eIF4G1 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-eIF4G1 Antibody

Description

Rabbit polyclonal antibody to eIF4G1.

Applications

WB, IHC, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).

Sequence

PEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, EIF4 gamma, EIF4F, EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, IF4G1_HUMAN, p220

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-eIF4G1 Antibody (A10162)

Western blot analysis of extracts of HeLa cells, using Anti-eIF4G1 Antibody (A10162) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-eIF4G1 Antibody (A10162)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-eIF4G1 Antibody (A10162) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-eIF4G1 Antibody (A10162)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-eIF4G1 Antibody (A10162). This Western blot was performed on the immunoprecipitate using Anti-eIF4G1 Antibody (A10162) at a dilution of 1:500.

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