Rabbit polyclonal antibody to eIF4EBP1 (phospho Thr45).
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100
Reactivity
Human
Immunogen
A phospho specific peptide corresponding to residues surrounding T45 of human Phospho-eIF4EBP1-T45.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
18 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Thr45) Antibody (A10899)
Western blot analysis of extracts from HeLa cells using Anti-eIF4EBP1 (phospho Thr45) Antibody (A10899). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
