Rabbit polyclonal antibody to eIF4EBP1 (phospho Thr36).
Applications
WB
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T36 of human eIF4EBP1 (NP_004086.1).
Sequence
YSTTP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
4E-BP1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Western Blot - Anti-eIF4EBP1 (phospho Thr36) Antibody (A16587)
Western blot analysis of extracts from MDA-MB-435 cells, using Anti-eIF4EBP1 (phospho Thr36) Antibody (A16587). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
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