Rabbit monoclonal [ARC53775] antibody to eIF4EBP1 (phospho Ser65).
Applications
WB
Dilutions
WB: 1:2,000-1:20,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S65 of human Phospho-EIF4EBP1-S65 (NP_004086.1).
Sequence
RNSPV
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC53775
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
15 - 20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Ser65) Antibody [ARC53775] (A308505)
Western blot analysis of 293T, using Anti-eIF4EBP1 (phospho Ser65) Antibody [ARC53775] (A308505) at 1:20,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Western Blot - Anti-eIF4EBP1 (phospho Ser65) Antibody [ARC53775] (A308505)
Western blot analysis of NIH/3T3, using Anti-eIF4EBP1 (phospho Ser65) Antibody [ARC53775] (A308505) at 1:20,000 dilution. NIH/3T3 cells were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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