Rabbit polyclonal antibody to eIF4EBP1 (phospho Ser65).
Applications
WB
Dilutions
WB: 1:500 - 1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
Phospho-specific peptide corresponding to residues surrounding Ser65 of human 4EBP1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.02% Sodium Azide.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) is also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent translation. Phosphorylation at serine 65 inhibits binding of 4EBP1 to eIF4F allowing cap-dependent translation.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
