Anti-eIF4E Antibody (A309356)

Name: Anti-eIF4E Antibody
Brand: Antibodies.com
SKU: A309356
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit polyclonal antibody to eIF4E for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-eIF4E Antibody

Description

Rabbit polyclonal antibody to eIF4E.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human eIF4E.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

29 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E_HUMAN, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

Enlarge Image

Figure 1: Western Blot - Anti-eIF4E Antibody (A309356)

Western blot analysis of various lysates, using Anti-eIF4E Antibody (A309356) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Western Blot - Anti-eIF4E Antibody (A309356)

Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using Anti-eIF4E Antibody (A309356) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-eIF4E Antibody (A309356)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-eIF4E Antibody (A309356) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-eIF4E Antibody (A309356)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-eIF4E Antibody (A309356) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.