Anti-eIF4E Antibody (A13922)

Name: Anti-eIF4E Antibody
Brand: Antibodies.com
SKU: A13922
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to eIF4E for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-eIF4E Antibody

Description

Rabbit polyclonal antibody to eIF4E.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).

Sequence

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E_HUMAN, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-eIF4E Antibody (A13922)

Western blot analysis of various lysates, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 2: Western Blot - Anti-eIF4E Antibody (A13922)

Western blot analysis of extracts from wild type(WT) and eIF4E knockout (KO) 293T(KO) cells, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 3: Immunohistochemistry - Anti-eIF4E Antibody (A13922)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-eIF4E Antibody (A13922)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.