Anti-eIF4A1 Antibody (A14643)

Name: Anti-eIF4A1 Antibody
Brand: Antibodies.com
SKU: A14643
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to eIF4A1 for WB, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-eIF4A1 Antibody

Description

Rabbit polyclonal antibody to eIF4A1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A1 (NP_001407.1).

Sequence

MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATP-dependent RNA helicase eIF4A-1, DDX2, DDX2A, eIF 4A I, eIF-4A-I, EIF4A, eIF4A I, eIF4A-I, Eukaryotic initiation factor 4A-I, Eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A, isoform 1, Eukaryotic translation initiation factor 4A1, IF4A1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-eIF4A1 Antibody (A14643)

Western blot analysis of various lysates, using Anti-eIF4A1 Antibody (A14643) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunofluorescence - Anti-eIF4A1 Antibody (A14643)

Immunofluorescence analysis of C6 cells using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 3: Immunofluorescence - Anti-eIF4A1 Antibody (A14643)

Immunofluorescence analysis of NIH/3T3 cells using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-eIF4A1 Antibody (A14643)

Immunofluorescence analysis of U2OS cells using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).