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Anti-eIF3B Antibody [ARC1448] (A309096)

Western Blot - Anti-eIF3B Antibody [ARC1448] (A309096) - Antibodies.com Immunofluorescence - Anti-eIF3B Antibody [ARC1448] (A309096) - Antibodies.com Immunofluorescence - Anti-eIF3B Antibody [ARC1448] (A309096) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC1448] antibody to eIF3B for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-eIF3B Antibody [ARC1448]
Description
Rabbit monoclonal [ARC1448] antibody to eIF3B.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 101-200 of human eIF3B (P55884).
Sequence
VPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1448
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
116 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
eIF-3-eta, eIF3 eta, eIF3 p110, eIF3 p116, EIF3-ETA, EIF3-P110, EIF3-P116, EIF3B_HUMAN, EIF3S9, EIF3S9, formerly, Eukaryotic translation initiation factor 3 subunit 9, eukaryotic translation initiation factor 3 subunit 9 eta, Eukaryotic translation initiation factor 3 subunit B, eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD), eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa, eukaryotic translation initiation factor 3, subunit 9, formerly, Eukaryotic translation initiation factor 3, subunit B, hPrt1, Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of, PRT1, Prt1 homolog
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-eIF3B Antibody [ARC1448] (A309096) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-eIF3B Antibody [ARC1448] (A309096)
Western blot analysis of extracts of various cell lines, using Anti-eIF3B Antibody [ARC1448] (A309096) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-eIF3B Antibody [ARC1448] (A309096) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-eIF3B Antibody [ARC1448] (A309096)
Immunofluorescence analysis of C6 cells using Anti-eIF3B Antibody [ARC1448] (A309096) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-eIF3B Antibody [ARC1448] (A309096) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-eIF3B Antibody [ARC1448] (A309096)
Immunofluorescence analysis of NIH-3T3 cells using Anti-eIF3B Antibody [ARC1448] (A309096) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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