Anti-eIF3B Antibody (A12865)

Name: Anti-eIF3B Antibody
Brand: Antibodies.com
SKU: A12865
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to eIF3B for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-eIF3B Antibody

Description

Rabbit polyclonal antibody to eIF3B.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human eIF3B (NP_003742.2).

Sequence

PVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

125 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

eIF-3-eta, eIF3 p110, eIF3 p116, EIF3S9, Eukaryotic translation initiation factor 3 subunit 9, Eukaryotic translation initiation factor 3 subunit B, hPrt1, Prt1 homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-eIF3B Antibody (A12865)

Western blot analysis of extracts of various cell lines, using Anti-eIF3B Antibody (A12865) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-eIF3B Antibody (A12865)

Immunofluorescence analysis of HeLa cells using Anti-eIF3B Antibody (A12865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-eIF3B Antibody (A12865)

Immunofluorescence analysis of NIH-3T3 cells using Anti-eIF3B Antibody (A12865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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