Anti-EIF2B5 Antibody (A12870)

Name: Anti-EIF2B5 Antibody
Brand: Antibodies.com
SKU: A12870
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to EIF2B5 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-EIF2B5 Antibody

Description

Rabbit polyclonal antibody to EIF2B5.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human eIF2B epsilon (eIF2B epsilon (EIF2B5)) (NP_003898.2).

Sequence

TLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

eIF2B GDP-GTP exchange factor subunit epsilon, EIF2BE, Translation initiation factor eIF2B subunit epsilon

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-EIF2B5 Antibody (A12870)

Western blot analysis of extracts of various cell lines, using Anti-EIF2B5 Antibody (A12870) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-EIF2B5 Antibody (A12870)

Immunofluorescence analysis of L929 cells using Anti-EIF2B5 Antibody (A12870) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-EIF2B5 Antibody (A12870)

Immunoprecipitation analysis of 200µg extracts of A-549 cells using 3µg eIF2B epsilon (eIF2B epsilon (EIF2B5)) antibody ( A10263). This Western blot was performed on the immunoprecipitate using Anti-EIF2B5 Antibody (A12870) at a dilution of 1:1000.

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