+1 (314) 370-6046 or
Contact Us
Explore our End of Year Promotion >>
Antibodies.com LogoAntibodies.com Logo
Sign In / RegisterMy Account
0

eIF2 alpha Cell Based ELISA Kit (A102879)

Western Blot - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.com Protocol Diagram - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.com Protocol Summary - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.com Western Blot - Control Antibody - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.com
$550
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
eIF2 alpha Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This eIF2 alpha Cell Based ELISA Kit allows for the detection of eIF2 alpha and the effects that certain stimulation conditions have on eIF2 alpha expression in different cell lines. Qualitative determination of eIF2 alpha concentration is achieved by an indirect ELISA format. In essence, the eIF2 alpha is captured by Anti-eIF2 alpha Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this eIF2 alpha Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
EIF 2, EIF 2 alpha, EIF 2A, EIF 2alpha, eIF-2-alpha, eIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit alpha, IF2A_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.comEnlarge Image
Figure 1: Western Blot - eIF2 alpha Cell Based ELISA Kit (A102879)
Western blot analysis of extracts from rat lung, using Anti-eIF2 alpha (Ab-51) Antibody.
Protocol Diagram - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.comEnlarge Image
Figure 2: eIF2 alpha Cell Based ELISA Kit (A102879)
A diagram showing the protocol for eIF2 alpha Cell Based ELISA Kit.
Protocol Summary - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.comEnlarge Image
Figure 3: eIF2 alpha Cell Based ELISA Kit (A102879)
A summary of the protocol for eIF2 alpha Cell Based ELISA Kit.
Western Blot - Control Antibody - eIF2 alpha Cell Based ELISA Kit (CB5226) - Antibodies.comEnlarge Image
Figure 4: Western Blot - eIF2 alpha Cell Based ELISA Kit (A102879)
The mouse monoclonal antibody to GAPDH is used as a positive control ineIF2 alpha Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using eIF2 alpha Cell Based ELISA Kit (A102879)? Please let us know so that we can list the citation on this page.
Main Menu Contact Us 0Checkout
Top