EdU Cell Proliferation Image Kit (Green Fluorescence) (A319642)

$205
Freight Charges
Lead Time
7-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
EdU Cell Proliferation Image Kit (Green Fluorescence)
Applications
Fluorescence Microscopy
Principle of Assay
This kit is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, and is complete within 30 minutes.
Components
EdU (10mM), BSA Wash Solution (5X), Fluor 488 azide, Reaction Buffer (10X), Copper Reagent, Reducing Agent, Hoechst 33342 (1000X), Hydroxyurea
Absorption Maxima
501 nm
Emission Maxima
525 nm
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using EdU Cell Proliferation Image Kit (Green Fluorescence) (A319642)? Please let us know so that we can list the citation on this page.
Top