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Anti-ECHS1 Antibody (A14476)

Western Blot - Anti-ECHS1 Antibody (A14476) - Antibodies.com Immunofluorescence - Anti-ECHS1 Antibody (A14476) - Antibodies.com Immunofluorescence - Anti-ECHS1 Antibody (A14476) - Antibodies.com Immunofluorescence - Anti-ECHS1 Antibody (A14476) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to ECHS1 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-ECHS1 Antibody
Description
Rabbit polyclonal antibody to ECHS1.
Applications
WB, ICC/IF
Dilutions
WB: 1:1,000-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 28-290 of human ECHS1 (NP_004083.3).
Sequence
ASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
31 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
C80529, EC 4.2.1.17, ECHS 1, Enoyl CoA hydratase 1, Enoyl CoA hydratase mitochondrial, Enoyl CoA hydratase short chain 1 mitochondrial, Enoyl Coenzyme A hydratase short chain 1, Enoyl Coenzyme A hydratase short chain 1 mitochondrial, Mitochondrial short chain enoyl coenzyme A hydratase 1, OTTHUMP00000020811, SCEH, Short chain enoyl CoA hydratase
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ECHS1 Antibody (A14476) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ECHS1 Antibody (A14476)
Western blot analysis of extracts of various cell lines, using Anti-ECHS1 Antibody (A14476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-ECHS1 Antibody (A14476) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-ECHS1 Antibody (A14476)
Immunofluorescence analysis of HeLa cells using Anti-ECHS1 Antibody (A14476) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-ECHS1 Antibody (A14476) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-ECHS1 Antibody (A14476)
Immunofluorescence analysis of MCF7 cells using Anti-ECHS1 Antibody (A14476) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-ECHS1 Antibody (A14476) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-ECHS1 Antibody (A14476)
Immunofluorescence analysis of NIH/3T3 cells using Anti-ECHS1 Antibody (A14476) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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