Anti-EAAT3 Antibody [S180-41] (A305150)

Name: Anti-EAAT3 Antibody [S180-41]
Brand: Antibodies.com
SKU: A305150
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S180-41] antibody to EAAT3 for WB, IP and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-EAAT3 Antibody [S180-41]

Description

Mouse monoclonal [S180-41] antibody to EAAT3.

Applications

WB, IP, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100,

Reactivity

Human, Rat

Immunogen

Fusion protein amino acids 1-524 (full length) of human EAAC1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S180-41

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EAA3_HUMAN, EAAC 1, EAAC1, EAAT 3, Excitatory amino acid carrier 1, Excitatory amino acid carrier1, Excitatory amino acid transporter 3, Excitatory amino acid transporter3, Excitatory amino-acid carrier 1, GLUTAMATE TRANSPORTER, HIGH-AFFINITY, MEAAC 1, MEAAC1, Neuronal and epithelial glutamate transporter, REAAC 1, REAAC1, Slc1 a1, Slc1a 1, SLC1A1, Sodium dependent glutamate/aspartate transporter 3, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1, SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1, Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1, Solute carrier family 1 member 1, Solute carrier family 1, member 1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-EAAT3 Antibody [S180-41] (A305150)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-EAAT3 Antibody [S180-41] (A305150), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) EAAC1 Antibody. (D) Composite.

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Figure 2: Western Blot - Anti-EAAT3 Antibody [S180-41] (A305150)

Western blot analysis of rat brain membrane lysate showing detection of EAAT3 protein using Anti-EAAT3 Antibody [S180-41] (A305150) at 1:1,000.

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Figure 3: Immunofluorescence - Anti-EAAT3 Antibody [S180-41] (A305150)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-EAAT3 Antibody [S180-41] (A305150), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) EAAC1 Antibody (D) Composite.

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