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Anti-ELF1 Antibody (A83273)

This product is identical to Abcam (ab11801). Discover more for less.

Western Blot - Anti-ELF1 Antibody (A83273) - Antibodies.com Western Blot - Anti-ELF1 Antibody (A83273) - Antibodies.com Immunohistochemistry - Anti-ELF1 Antibody (A83273) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to ELF1 for ELISA, WB and IHC.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-ELF1 Antibody
Description
Goat polyclonal antibody to ELF1.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:64,000, WB: 0.03-0.1µg/ml, IHC: 2.5µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the C terminal region of human ELF1.
Sequence
C-AMKQNELLEPNSF
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
65 kDa
Predicted MW
64.9kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
E74 like factor 1, E74-like factor 1, ELF 1, ELF1_HUMAN, Ets domain transcription factor, ETS related transcription factor Elf 1, ETS related transcription factor Elf1, ets-related transcription factor, ETS-related transcription factor Elf-1, mElf 1, p70, Sts1, TRANSCRIPTION FACTOR ELF 1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ELF1 Antibody (A83273) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ELF1 Antibody (A83273)
ELF1 expression in Human Lymph Node (A), Human Tonsil (B), and Mouse Thymus (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-ELF1 Antibody (A83273) at 0.3µg/ml and detected by chemiluminescence.
Western Blot - Anti-ELF1 Antibody (A83273) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-ELF1 Antibody (A83273)
ELF1 expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-ELF1 Antibody (A83273) at 0.1µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-ELF1 Antibody (A83273) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-ELF1 Antibody (A83273)
ELF1 expression in Human Thymus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ELF1 Antibody (A83273) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
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