Western blot analysis of extracts of MCF-7 cells, using Anti-E2F6 Antibody (A89621) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-E2F6 Antibody (A89621). This Western blot was performed on the immunoprecipitate using Anti-E2F6 Antibody (A89621) at a dilution of 1:500.
Chromatin immunoprecipitation (ChIP) analysis of extracts of 293T cells, using Anti-E2F6 Antibody (A89621) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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