Anti-E2F6 Antibody (A15091)

Name: Anti-E2F6 Antibody
Brand: Antibodies.com
SKU: A15091
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to E2F6 for WB, IHC, IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-E2F6 Antibody

Description

Rabbit polyclonal antibody to E2F6.

Applications

WB, IHC, IF, IP, ChIP

Dilutions

WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:20-1:100, IP: 1:50-1:100, ChIP:: 1:20-1:50

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).

Sequence

MSQQRPARKLPSLLLDPTEETVRRRCRDPINVEGLLPSKIRINLEDNVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYDITNVLDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E2F 6, E2F binding site modulating activity protein, E2F transcription factor 6, E2F transcription factor 6 isoform 1, E2F-6, E2F6_HUMAN, EMA, MGC111545, Transcription factor E2F6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-E2F6 Antibody (A15091)

Western blot analysis of extracts of various cell lines, using Anti-E2F6 Antibody (A6151) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-E2F6 Antibody (A15091)

Immunohistochemistry of paraffin-embedded mouse lung using Anti-E2F6 Antibody (A6151) at dilution of 1:200 (40x lens).

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Figure 3: Immunofluorescence - Anti-E2F6 Antibody (A15091)

Immunofluorescence analysis of U2OS cells using Anti-E2F6 Antibody (A6151). Blue: DAPI for nuclear staining.

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Figure 4: Immunoprecipitation - Anti-E2F6 Antibody (A15091)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg Anti-E2F6 Antibody (A6151). Western blot was performed from the immunoprecipitate using Anti-E2F6 Antibody (A6151) at a dilition of 1:500.