Recombinant Anti-E2F4 Antibody [E2F4/8645R] (A314902)

Anti-E2F4 Antibody [E2F4/8645R]

Recombinant rabbit monoclonal [E2F4/8645R] antibody to E2F4.

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.

Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

IHC: 1-2 µg/ml

Human

Recombinant fragment (around Amino Acids 1-100) of human E2F4 protein. The exact sequence is proprietary.

Rabbit

Monoclonal

E2F4/8645R

kappa

Unconjugated

Protein A/G chromatography.

200 µg/ml

60 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

E2F 4, E2F transcription factor 4, E2F transcription factor 4 p107/p130 binding, E2F-4, E2F4_HUMAN, p107/p130 binding protein, Transcription factor E2F 4, Transcription factor E2F4