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Dyskerin Cell Based ELISA Kit (A102965)

Western Blot - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.com Protocol Diagram - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.com Protocol Summary - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.com Western Blot - Control Antibody - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.com
$550
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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Dyskerin Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Dyskerin Cell Based ELISA Kit allows for the detection of Dyskerin and the effects that certain stimulation conditions have on Dyskerin expression in different cell lines. Qualitative determination of Dyskerin concentration is achieved by an indirect ELISA format. In essence, the Dyskerin is captured by Anti-Dyskerin Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Dyskerin Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
CBF5, CBF5 homolog, Cbf5p homolog, DKC, DKC 1, DKC1, DKC1_HUMAN, DKCX, Dyskeratosis congenita 1, Dyskeratosis congenita 1 dyskerin, H/ACA ribonucleoprotein complex subunit 4, NAP 57, NAP57, NOLA 4, NOLA4, Nopp140 associated protein of 57 kDa, Nopp140-associated protein of 57 kDa, Nucleolar protein family A member 4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP 101, XAP101
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Dyskerin Cell Based ELISA Kit (A102965)
Western blot analysis of extracts from JurKat cells, using Anti-Dyskerin Antibody.
Protocol Diagram - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.comEnlarge Image
Figure 2: Dyskerin Cell Based ELISA Kit (A102965)
A diagram showing the protocol for Dyskerin Cell Based ELISA Kit.
Protocol Summary - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.comEnlarge Image
Figure 3: Dyskerin Cell Based ELISA Kit (A102965)
A summary of the protocol for Dyskerin Cell Based ELISA Kit.
Western Blot - Control Antibody - Dyskerin Cell Based ELISA Kit (CB5795) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Dyskerin Cell Based ELISA Kit (A102965)
The mouse monoclonal antibody to GAPDH is used as a positive control inDyskerin Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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